Phenotypic Analysis of Antigen-Specific T Lymphocytes
John D. Altman, Paul A. H. Moss, Philip Goulder, Dan H. Barouch, Michael G. McHeyzer‐Williams, John I. Bell, Andrew James McMichael, Mark M. Davis
Science · 1996 · 3,588 citations
Abstract
Identification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tetramers. Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. In general, tetramer binding correlated well with cytotoxicity assays. This approach should be useful in the analysis of T cells specific for infectious agents, tumors, and autoantigens.
Cite this paper
Altman, J. D., Moss, P. A. H., Goulder, P., Barouch, D. H., McHeyzer‐Williams, M. G., Bell, J. I., McMichael, A. J., & Davis, M. M. (1996). Phenotypic analysis of antigen-specific T lymphocytes. Science, 274(5284), 94–96. https://doi.org/10.1126/science.274.5284.94
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