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Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci

Srdjan Stepanović, Dragana Vuković, Veronika Holá, Giovanni Di Bonaventura, Slobodanka Djukić, Ivana B. Cirkovic, Filip Růžička

Apmis · 2007 · 2,535 citations

Abstract

The details of all steps involved in the quantification of biofilm formation in microtiter plates are described. The presented protocol incorporates information on assessment of biofilm production by staphylococci, gained both by direct experience as well as by analysis of methods for assaying biofilm production. The obtained results should simplify quantification of biofilm formation in microtiter plates, and make it more reliable and comparable among different laboratories.

Cite this paper

Stepanović, S., Vuković, D., Holá, V., Bonaventura, G. D., Djukić, S., Cirkovic, I. B., & Růžička, F. (2007). Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. Apmis, 115(8), 891–899. https://doi.org/10.1111/j.1600-0463.2007.apm_630.x

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