Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton

Abstract

Using a ternary gradlent s}istem, over 50 carotenoids, chlorophylls and their denvatives were separated from marine phytoplankton.Only 2 palrs of carotenoid pigments (19'-butanoyloxyfucoxanthin and siphonaxanthin, and 19'-hexanoyloxyfucoxanthin and 9'-cis-neoxanthln) and 3 chlorophylls (chlorophylls c , , c2 and Mg 2.4 divinyl pheoporphyrin a5 monomethyl ester [Mg2,4D]) were not resolved.Pigment chromatograms are presented for 12 uniaigal cultures from 10 algal classes important in the marine environment: Alnphidinlum cal-terae Hulbert (Dinophyceae), Chroomonas salina (Wislouch) Butcher (Cryptophyceae), Dunallella tertrolecta Butcher (Chlorophyceae); Emil~anla huxleyi (Lohmann) Hay et Mohler and Pavlova lutheri (Droop) Green (Prymnesiophyceae); Euglena gracllis Klebs (Euglenophyceae); Micl-omonas pusllla (Butcher) Manton et Parke and Pycnococcus provasohi Guillard (Praslnophyceae); Pelagococcus subv~ridis Norris (Chrysophyceae); Phaeodactylum tncornutum Bohlln (Baclllanophyceae); Porphyndlum cruentum (Bory) Drew et Ross (Rhodophyceae), and Synechococcus sp (Cyanophyceae).A chromatogram IS also given of a complex mixture of over 50 algal pigments such as might be found in a phytoplankton field sample This method is useful for analysis of phytoplankton plgments in seawater samples and other Instances where separations of complex pigment mixtures are required

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